This project is a collaboration with C. Merlin from LCPME (CNRS UMR 7564) on the processing of antibiograms. Our contribution mostly focuses on the automation of the antibiogram analysis using OpenCV. Our input images come from a highly sensitive CCD Camera (Berthold Nightshade) which can detect the bioluminescence generated by bacteria. The images below show typical images (normal image on the left, high sensitivity bioluminescence on the right).

From the detection of the disk distribution on the left, we can reconstruct the antibiotic effect on the bioluminescence on the right:

Bibliography

  1. Guilloteau, H., Pradalier, C., Roman, V. L., Bellanger, X., Billard, P., & Merlin, C. (2022). Identification of antibiotics triggering the dissemination of antibiotic resistance genes by SXT/R391 elements using a dedicated high-throughput whole-cell biosensor assay. Journal of Antimicrobial Chemotherapy, 77(1), 112–123. PDF
  2. Bellanger, X., Pradalier, C., Guilloteau, H., Roman, V., & Merlin, C. (2019). Identification d’antibiotiques stimulant la dissémination de gènes d’antibiorésistance en concentrations sub-inhibitrices et conséquences au niveau environnemental. IXe Colloque De l’Association Francophone d’Ecologie Microbienne.
  3. Bellanger, X., Pradalier, C., Guilloteau, H., Roman, V., & Merlin, C. (2019). When antibiotics stimulate the spread of resistance genes: small doses and dramatic effects. 15e Congrès National De La Societe Française De Microbiologie.
  4. Bellanger, X., Pradalier, C., Guilloteau, H., Barrón, M. de L. C., Roman, V., & Merlin, C. (2018). Identifying antibiotics triggering the dissemination of resistance genes at low concentrations. Xenowac II.
  5. Scornec, H., Guilloteau, H., Groshenry, G., Pradalier, C., Bellanger, X., & Merlin, C. (2017). Exploring the effect of antibiotics at sub-MIC level on the activity of mobile genetic elements. 4th EDAR.
  6. Scornec, H., Guilloteau, H., Groshenry, G., Pradalier, C., Bellanger, X., & Merlin, C. (2017). When sub-inhibitory concentrations of antibiotics promote the dissemination of unselected resistance genes. FEMS 2017.